In vitro antioxidant activity of Lantadene A

1Chong Grace-Lynn, 2Ibrahim Darah and 1Sasidharan Sreenivasan

1Institute for Research In Molecular Medicine, Universiti Sains Malaysia, 11800 USM, Pulau Pinang, Malaysia.2School of Biological Sciences, Universiti Sains Malaysia, 11800 USM, Pulau Pinang, Malaysia.Corresponding Author: srisasidharan@yahoo.com

Abstract

Lantadenes are the pentacyclic triterpenoids present in the leaves of the plant Lantana camara. Pentacyclic triterpenes are often studied as their biological properties are considerable andcontributes to the development of modern therapeutic drugs. In recent years various natural product based compounds are extensively studied for various pharmacological activities including antioxidant activity. Therefore, this study aims to determine the in vitro antioxidant activity of Lantadene A (from Lantana camara ) as results will enhance the knowledge of the compound towards development of hepatoprotective agents. The antioxidant assays performed on the compound were the DPPH radical scavenging assay, nitric oxide assay, superoxide anions scavenging activity and iron chelating assay. Results showed promising antioxidant activities as the IC50 for Lantadene A in the above assays were 6.574 mg/ml, 0.098 mg/ml, 2.506 mg/ml and 0.001 mg/ml respectively as compared to the standards (BHT, ascorbic acid and EDTA) 0.027 mg/ml, 0.075 mg/ml, 1.025 mg/ml and 0.47 mg/ml respectively. The DPPH radical scavenging assay for Lantadene A was weaker than BHT while iron chelating assay of Lantadene A is much stronger than the standard. The rest of the assays were comparable to the reference standards. These results suggest that Lantadene A can be a potential cadidate to be developed as an antioxidant.

Keywords: Lantadene A, Antioxidant activity, triterpenoids, free radicals

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