Antimicrobial Guided Fractionation of Phenolic Compounds from Baccaurea motleyana
Nurul Shafiqah Hashim1, Ahmad Rosma2,
Shaida Fariza Sulaiman1
aSchool of Biological Sciences, Universiti Sains Malaysia, 11800 USM, Pulau Pinang, Malaysia.b School of Industrial Technology, Universiti Sains Malaysia, 11800 USM, Pulau Pinang, Malaysia
Baccaurea motleyana (Rambai), Baccaurea polyneura (Jentik) and Baccaurea macrocarpa (Tampoi)that belong to the Phyllanthaceae family, were selected in this study. Three solvents of varying polarities (petroleum ether, ethyl acetate and methanol) were used to sequentially extract the compounds from various parts of these plants (peels, leaves, seeds, pulps, peduncles and branches). All ethyl acetate and methanol extracts, as well as their juices and freeze dried juices were then evaluated for their antimicrobial activities against skin diseases microbials. The minimum inhibitory concentration (MIC) for crude extracts was ranging from 125 µg/mL to 1000 µg/mL. Ethyl acetate crude extract of B. motleyana’s peel with 2.74±1.38 µg GAE/mg extracts of total phenolic content gave the best result and was fractionated. The fractions were then tested against the same microorganisms and the results showed that fraction 2 and fraction 4 showed the best results. The fractions were selected for their chemical profiling by using Ultra Performance Liquid Chromatography (UPLC).The phenolics compounds in fraction 2 were identified as isoflavone derivatives while fraction 4 was comprised of phenolic acid and caffeic acid derivatives. Therefore, the results revealed the contribution of phenolic compounds to their antimicrobial activities.
Keywords: Inhibitor, phenolics, chromatography